The migration speed of cancer cells influenced by macrophages and myofibroblasts co-cultured in a microfluidic chip

  • Hsu T
  • Kao Y
  • Lin W
 et al. 
  • 55


    Mendeley users who have this article in their library.
  • 17


    Citations of this article.


We employ a microfluidic chip with three culture chambers to investigate the interactions among lung cancer cells, macrophages and myofibroblasts. By mixing the conditioned media of macrophages and myofibroblasts in this chip, we confirm that these two stromal cells have synergistic effects in accelerating the migration of cancer cells. However, as the myofibroblasts are pretreated with the conditioned medium of macrophages, the myofibroblasts' ability to enhance the migration of cancer cells is lowered. The tumour necrosis factor-α produced by macrophages reduces the expression of α-smooth muscle actin and the secretion of transforming growth factor-β1 in myofibroblasts. Once the tumour necrosis factor-α in the macrophage conditioned medium is neutralized, the macrophage medium-pretreated myofibroblasts can still accelerate the migration of cancer cells.

Get free article suggestions today

Mendeley saves you time finding and organizing research

Sign up here
Already have an account ?Sign in

Find this document


  • Tsi Hsuan Hsu

  • Yi Lun Kao

  • Wei Liang Lin

  • Jian Long Xiao

  • Po Ling Kuo

  • Chih Wei Wu

Cite this document

Choose a citation style from the tabs below

Save time finding and organizing research with Mendeley

Sign up for free