Mining novel biomarkers for prognosis of gastric cancer with serum proteomics

  • Qiu F
  • Yu J
  • Chen Y
 et al. 
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Abstract

BACKGROUND: Although gastric cancer (GC) remains the second cause of cancer-related death, useful biomarkers for prognosis are still unavailable. We present here the attempt of mining novel biomarkers for GC prognosis by using serum proteomics. METHODS: Sera from 43 GC patients and 41 controls with gastritis as Group 1 and 11 GC patients as Group 2 was successively detected by Surface Enhanced Laser Desorption/ionization Time of Flight Mass Spectrometry (SELDI-TOF-MS) with Q10 chip. Peaks were acquired by Ciphergen ProteinChip Software 3.2.0 and analyzed by Zhejiang University-ProteinChip Data Analysis System (ZJU-PDAS). CEA level were evaluated by chemiluminescence immunoassay. RESULTS: After median follow-up periods of 33 months, Group 1 with 4 GC patients lost was divided into 20 good-prognosis GC patients (overall survival more than 24 months) and 19 poor-prognosis GC patients (no more than 24 months). The established prognosis pattern consisted of 5 novel prognosis biomarkers with 84.2% sensitivity and 85.0% specificity, which were significantly higher than those of carcinoembryonic antigen (CEA) and TNM stage. We also tested prognosis pattern blindly in Group 2 with 66.7% sensitivity and 80.0% specificity. Moreover, we found that 4474-Da peak elevated significantly in GC and was associated with advanced stage (III+IV) and short survival (p < 0.03). CONCLUSION: We have identified a number of novel biomarkers for prognosis prediction of GC by using SELDI-TOF-MS combined with sophisticated bioinformatics. Particularly, elevated expression of 4474-Da peak showed very promising to be developed into a novel biomarker associated with biologically aggressive features of GC.

Author-supplied keywords

  • Area Under Curve
  • Carcinoembryonic Antigen/blood
  • Humans
  • Luminescence
  • Neoplasm Staging
  • Prognosis
  • Protein Array Analysis
  • Proteomics/*methods
  • ROC Curve
  • Reproducibility of Results
  • Sensitivity and Specificity
  • Spectrometry, Mass, Matrix-Assisted Laser Desorpti
  • Stomach Neoplasms/*blood/genetics/pathology
  • Tumor Markers, Biological/*blood

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Authors

  • F M Qiu

  • J K Yu

  • Y D Chen

  • Q F Jin

  • M H Sui

  • J Huang

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