Mismatched appositions of presynaptic and postsynaptic components in isolated hippocampal neurons.

  • Rao A
  • Cha E
  • Craig A
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Abstract

To determine whether presynaptic input is necessary for postsynaptic differentiation, we isolated hippocampal neurons in microisland culture and thus deprived pyramidal cells of GABA input and GABAergic neurons of glutamate input. We find that glutamate input is necessary for clustering the AMPA-type glutamate receptor but not for clustering the NMDA receptor or the associated PSD-95 family scaffold in GABAergic cells; GABA input is not necessary for clustering the GABA(A) receptor or gephyrin in pyramidal cells. Isolated neurons showed a surprising mismatch of presynaptic and postsynaptic components. For example, in isolated pyramidal neurons, although GABA(A) receptor clusters covered

Author-supplied keywords

  • AMPA
  • AMPA: antagonists & inhibitors
  • AMPA: metabolism
  • Animals
  • Carrier Proteins
  • Carrier Proteins: metabolism
  • Cell Differentiation
  • Cell Differentiation: physiology
  • Cells
  • Cultured
  • Excitatory Postsynaptic Potentials
  • Excitatory Postsynaptic Potentials: drug effects
  • Excitatory Postsynaptic Potentials: physiology
  • GABA-A
  • GABA-A Receptor Antagonists
  • GABA-A: metabolism
  • Glutamate
  • Glutamate Decarboxylase
  • Glutamate Decarboxylase: metabolism
  • Glutamate: metabolism
  • Glutamic Acid
  • Glutamic Acid: metabolism
  • Glutamic Acid: pharmacology
  • Hippocampus
  • Hippocampus: cytology
  • Hippocampus: drug effects
  • Hippocampus: metabolism
  • Hippocampus: radionuclide imaging
  • Intracellular Signaling Peptides and Proteins
  • Membrane Proteins
  • Membrane Proteins: metabolism
  • N-Methyl-D-Aspartate
  • N-Methyl-D-Aspartate: antagonists & inh
  • N-Methyl-D-Aspartate: metabolism
  • Nerve Tissue Proteins
  • Nerve Tissue Proteins: metabolism
  • Neural Inhibition
  • Neural Inhibition: physiology
  • Neurons
  • Neurons: cytology
  • Neurons: drug effects
  • Neurons: metabolism
  • Pyramidal Cells
  • Pyramidal Cells: cytology
  • Pyramidal Cells: drug effects
  • Pyramidal Cells: metabolism
  • Rats
  • Receptor Aggregation
  • Receptor Aggregation: drug effects
  • Receptor Aggregation: physiology
  • Receptors
  • Synapses
  • Synapses: metabolism
  • Synaptophysin
  • Synaptophysin: metabolism
  • gamma-Aminobutyric Acid
  • gamma-Aminobutyric Acid: metabolism
  • gamma-Aminobutyric Acid: pharmacology

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Authors

  • A Rao

  • E M Cha

  • a M Craig

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