Mitochondrial membrane potential and glutamate excitotoxicity in cultured cerebellar granule cells

  • Ward M
  • Rego A
  • Frenguelli B
 et al. 
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Abstract

The relationship between changes in mitochondrial membrane potential (Deltapsi(m)) and the failure of cytoplasmic Ca(2+) homeostasis, delayed Ca(2+)deregulation (DCD), is investigated for cultured rat cerebellar granule cells exposed to glutamate. To interpret the single- cell fluorescence response of cells loaded with tetramethylrhodamine methyl ester (TMRM(+)) or rhodamine-123, we devised and validated a mathematical simulation with well characterized effectors of Deltapsi(m) and plasma membrane potential (Deltapsi(P)). Glutamate usually caused an immediate decrease in Deltapsi(m) of

Author-supplied keywords

  • *Cytoplasmic Granules
  • Action Potentials/drug effects
  • Adenosine Triphosphate/metabolism
  • Animal
  • Antibiotics, Macrolide/pharmacology
  • Antimycin A/pharmacology
  • Calcium/metabolism
  • Cells, Cultured
  • Cerebellum/cytology/drug effects/*metabolism
  • Enzyme Inhibitors/pharmacology
  • Fluorescent Dyes
  • Glutamic Acid/*metabolism/pharmacology
  • Intracellular Membranes/*metabolism
  • Membrane Potentials/drug effects
  • Mitochondria/*metabolism
  • Potassium Chloride/metabolism/pharmacology
  • Rats
  • Rats, Wistar
  • Receptors, Glutamate/metabolism
  • Rhodamines
  • Uncoupling Agents/pharmacology

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  • SCOPUS: 2-s2.0-0034307759
  • PUI: 30747415
  • ISSN: 02706474
  • PMID: 11007877
  • ISBN: 0270-6474
  • SGR: 0034307759

Authors

  • M W Ward

  • A C Rego

  • B G Frenguelli

  • D G Nicholls

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