Models for lymphoma.

  • Biragyn A
  • Kwak L
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This unit describes experimental procedures for development of therapeutic vaccines, particularly "second-generation" recombinant vaccines. Specifically, a general procedure for handling and culturing lymphoma cell lines in vitro and their subsequent challenge into syngeneic mice is described. Several protocols describe the production of various idiotype (Id)-based or cellular lymphoma vaccine formulations. In particular, the novel approach of rendering nonimmunogenic lymphoma-derived scFv or Id immunogenic by fusing it with a chemokine moiety is described. As an alternative, a protocol for expression and purification of these chemokine-fusion proteins from E. coli is included. A general procedure is included for cloning of cytokine genes, for example, murine GM-CSF, in A20 lymphoma cells. Support protocols are presented for chemically conjugating intact Ig protein with KLH to produce a prototype protein vaccine and using the Helios Gene Gun System to immunize mice with recombinant DNA tumor vaccines.

Author-supplied keywords

  • Animals
  • Antibodies, Anti-Idiotypic
  • Antibodies, Anti-Idiotypic: immunology
  • Cell Line, Tumor
  • Disease Models, Animal
  • Enzyme-Linked Immunosorbent Assay
  • Granulocyte-Macrophage Colony-Stimulating Factor
  • Granulocyte-Macrophage Colony-Stimulating Factor:
  • Hemocyanin
  • Hemocyanin: immunology
  • Immunoglobulin Variable Region
  • Immunoglobulin Variable Region: genetics
  • Immunoglobulin Variable Region: immunology
  • Immunotherapy
  • Lymphoma
  • Lymphoma: prevention & control
  • Mice
  • Neoplasm Transplantation
  • Protein Folding
  • Recombinant Fusion Proteins
  • Recombinant Fusion Proteins: immunology
  • Vaccines, DNA

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  • Arya Biragyn

  • Larry W Kwak

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