Molecular characterization of Clostridium tetani strains by pulsed-field gel electrophoresis and colony PCR

  • Plourde-Owobi L
  • Seguin D
  • Baudin M
 et al. 
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Pulsed-field gel electrophoresis and PCR were applied for the first time to the molecular characterization of Clostridium tetani. Among five strains tested, one (CN1339) turned out to contain a mixture of two genetically different clones and two (D11 and G761) to contain bacteria differing by the presence or absence of the 74-kb plasmid harboring the tetX gene.

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  • Lucile Plourde-Owobi

  • Delphine Seguin

  • Marie Anne Baudin

  • Catherine Moste

  • Bachra Rokbi

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