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Journal article

Molecular cloning and functional characterization of a novel decarboxylase from uncultured microorganisms

Jiang C, Wu B ...see all

Biochem Biophys Res Commun, vol. 357, issue 2 (2007) pp. 421-426

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Abstract

The metagenomic library approach has been used successfully to isolate novel biocatalyst genes from uncultured microorganisms. We report the cloning of a novel decarboxylase gene by sequence-based screening of a plasmid metagenomic library constructed with DNA from alkaline polluted soils. The gene was named undec1 A and had an open reading frame of 1077 base pairs. It encoded a 359 amino acid polypeptide with a molecular mass of 38 kDa. The predicted protein had 58% similarity to a decarboxylase from Chlorobium phaeobacteroides BS1. The putative decarboxylase gene was subcloned into pETBlue-2 vector and overexpressed in Escherichia coli Tuner (DE3) pLac. The recombinant protein was purified to homogeneity. Functional characterization with liquid chromatography-mass spectrometry confirmed that the recombinant Undec1 A protein catalyzed the decarboxylation of L-cysteine to form cysteamine.

Author-supplied keywords

  • Amino Acid Sequence
  • Carboxy-Lyases/*chemistry/genetics/*metabolism
  • Cell Culture Techniques
  • Cloning, Molecular/*methods
  • Enzyme Activation
  • Enzyme Stability
  • Escherichia coli/*genetics/*metabolism
  • Molecular Sequence Data
  • Molecular Weight
  • Recombinant Proteins/chemistry/metabolism
  • Sequence Homology, Amino Acid

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Authors

  • C Jiang

  • B Wu

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