Monitoring the expression profiles of 7000 Arabidopsis genes under drought, cold and high-salinity stresses using a full-length cDNA microarray

  • Seki M
  • Narusaka M
  • Ishida J
 et al. 
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Abstract

Full-length cDNAs are essential for functional analysis of plant genes . Recently , cDNA microarray analysis has been developed for quantitative analysis of global and simultaneous analysis of expression profiles . Microarray technology is a powerful tool for identifying genes induced by environmental stimuli or stress and for analyzing their expression profiles in response to environmental signals . We prepared an Arabidopsis full-length cDNA microarray containing around 7 , 000 independent full-length cDNA groups and analyzed the expression profiles of genes . The transcripts of 245 , 299 , 54 and 213 genes increased after abscisic acid ( ABA ) , drought- , cold- , and salt-stress treatments , respectively , with inducibilities more than fivefold compared with those of control genes [ corrected ] . The cDNA microarray analysis showed that many ABA-inducible genes were induced after drought- and high-salinity-stress treatments , and that there is more crosstalk between drought and ABA responses than between ABA and cold responses . Among the ABA-inducible genes identified , we identified 22 transcription factor genes , suggesting that many transcriptional regulatory mechanisms exist in the ABA signal transduction pathways .

Author-supplied keywords

  • Abiotic stress
  • Arabidopsis thaliana
  • CDNA microarray
  • Full-length cDNA

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Authors

  • Motoaki Seki

  • Mari Narusaka

  • Junko Ishida

  • Tokihiko Nanjo

  • Miki Fujita

  • Youko Oono

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