Morphological characterization of Cupriavidus necator DSM 545 flocs through image analysis

  • Finkler L
  • Ginoris Y
  • Luna C
 et al. 
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Abstract  Flocculating agents are used as auxiliary to recover
bacterial cells in downstream processes for polyhydroxyalkanoate
production. However little is known about the Curpiavidus necator
flocs. In this work a new procedure for floc characterization through
digital image analysis is presented and validated using the batch
settling test. Average diameter, particle size distribution and morphological
characteristics of the microbial aggregates were obtained from the
flocculation/sedimentation process of the Cupriavidus necator
DSM 545 cells by the use of tannin as flocculating agent. The experimental
results demonstrated that the proposed method is adequate to determine
the average floc diameter with values around 150 ?m in accordance
with the value obtained from the batch settling test. Nevertheless
a morphological characterization of Cupriavidus necator DSM
545 bioaggregates in terms of size distribution and regularity could
only be performed by an image analysis procedure. The procedure allowed
us to describe the regularity of bacterial flocs through the quantification
of morphological parameters of Euclidean [convexity (Conv) and form
factor (FF)] and fractal geometry [surface fractal dimension (D BS)],
which are important factors to be considered in the settling efficiency
of aggregates.

Author-supplied keywords

  • Aggregation
  • Bioseparations
  • Cupriavidus necator
  • Downstream processing
  • Image analysis

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  • L. Finkler

  • Y. P. Ginoris

  • C. L. Luna

  • T. L. Alves

  • J. C. Pinto

  • M. A.Z. Coelho

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