Morphological and Molecular Identification of Colletotrichum acutatum from Tomato Fruit

  • Živković S
  • Stojanović S
  • Ivanović Ž
 et al. 
  • 38

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Abstract

Colletotrichum gloeosporioides, Colletotrichum acutatum, Colletotrichum coccodes, and Colletotrichum dematium are the four main species of Colletotrichum that cause tomato anthracnose. In Serbia, the occurrence of anthracnose on tomato fruit has been record- ed during the last several years. Typical fruit symptoms include dark, sunken, and circu- lar lesion with orange conidial masses. Pathogen isolates were obtained from a diseased tomato fruits, on PDA medium forming a white to gray colonies. The cultures developed black acervuli around the center of the colony. Conidia were hyaline, aseptate, and fusi- form or rarely cylindrical. Appressoria were smooth, simple, clavate to ovate, and varied from light to dark brown. Pathogenicity tests with representative isolates were conducted on symptomless, detached tomato fruits. All tested isolates caused anthracnose lesions on tomato fruit after 7 days of incubation. Koch’s postulates were fulfilled by reisolation from inoculated tomato fruits. PCR analysis (using species-specific primer pair, CaInt2/ ITS4) of genomic DNA from tomato isolates resulted in an amplification product of 490 bp, specific for C. acutatum, further confirming the identity of the pathogen. Based on morphological and molecular characteristics, the isolates from tomato fruit were deter- mined as C. acutatum. Keywords:

Author-supplied keywords

  • anthracnose
  • colletotrichum acutatum
  • identification
  • tomato

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Authors

  • Svetlana Živković

  • Saša Stojanović

  • Žarko Ivanović

  • Nenad Trkulja

  • Nenad Dolovac

  • Goran Aleksic

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