Morphological and physiological responses of proliferating shoots of teak to temporary immersion and BA treatments

  • Quiala E
  • Cañal M
  • Meijón M
 et al. 
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Shoot-tips, collected from greenhouse-grown plants of Tectona grandis L. (teak), were incubated on a semi-solidMurashige andSkoog (MS)mediumwith2%(w/v) sucrose, and supplemented with 4.44 lM 6-benzyladenine (BA). These were then transferred to a temporary immersion system (TIS) using liquid MS medium supplemented with 0 (CK-free medium), 2.22, 4.44, 6.66 lM BA. High mean numbers of shoots per explant were obtained when explants were grown on medium containing either 4.44 or 6.66 lM BA and yielding 7.7 and 10.3 normal shoots (NS)/explant, respectively. Moreover, these high BA levels contributed to lower accumulation of phenolic compounds and deposition of lignin in vascular cells of the teak shoots following histo- chemical analysis. Morphological analysis of proliferating shoots by scanningmicroscopy revealed that leaves of shoots incubated on either CK-free medium, 2.22, or 4.44 lMBA had elliptical stomata; whereas, stomata of leaves of shoots grown on medium containing 6.66 lM BA were primarily ring-shaped, raised, and open. Moreover, misshapen stomata with broken epidermal layers of guard cells, typical of hyperhydric leaves, were also observed. When shoots were

Author-supplied keywords

  • Cytokinin
  • Forest plant
  • Hyperhydricity
  • Liquid medium
  • Micropropagation
  • Semi-automation

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