Multilocus sequence typing of Staphylococcus aureus with DNA array technology

  • van Leeuwen W
  • Jay C
  • Snijders S
 et al. 
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Abstract

A newly developed oligonucleotide array suited for multilocus sequence typing (MLST) of Staphylococcus aureus strains was analyzed with two strain collections in a two-center study. MLST allele identification for the first strain collection fully agreed with conventional strain typing. Analysis of strains from the second collection revealed that chip-defined MLST was concordant with conventional MLST. Array-mediated MLST data were reproducible, exchangeable, and epidemiologically concordant.

Author-supplied keywords

  • *Sequence Analysis, DNA
  • Alleles
  • Bacterial Proteins/*genetics
  • Bacterial Typing Techniques
  • Humans
  • Methicillin Resistance
  • Oligonucleotide Array Sequence Analysis/*methods
  • Staphylococcal Infections/microbiology
  • Staphylococcus aureus/*classification/drug effects

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Authors

  • W B van Leeuwen

  • C Jay

  • S Snijders

  • N Durin

  • B Lacroix

  • H A Verbrugh

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