Multiparameter imaging for the analysis of intracellular signaling

  • Schultz C
  • Schleifenbaum A
  • Goedhart J
 et al. 
  • 4

    Readers

    Mendeley users who have this article in their library.
  • N/A

    Citations

    Citations of this article.

Abstract

In biological experimentation and especially in drug discovery there is a trend towards more complex test systems. Cell-based assays are replacing conventional binding or enzyme assays more and more. This development is strongly driven by novel fluorescent probes that give insight into cellular processes. Target proteins are studied in their natural environment; this gives much more realistic test results, especially with respect to enzyme location and kinetics. However, in the complex environment of cells, many parameters contribute to the performance of the protein of interest. Therefore, it would be desirable to monitor simultaneously as many of the relevant cellular processes as possible. Here, we discuss the possibilities and limitations provided by multiparameter monitoring of cellular events with fluorescent probes. Some novel examples of the use of fluorescent probes and multiparameter imaging are shown.

Author-supplied keywords

  • Fluorescence Resonance Energy Transfer
  • Fluorescent Dyes/*analysis/metabolism
  • Microscopy, Fluorescence/*methods
  • Models, Molecular
  • Protein Kinase C/metabolism
  • Signal Transduction/*physiology
  • Spectrometry, Fluorescence/*methods

Get free article suggestions today

Mendeley saves you time finding and organizing research

Sign up here
Already have an account ?Sign in

Find this document

Authors

  • C Schultz

  • A Schleifenbaum

  • J Goedhart

  • T W Gadella Jr.

Cite this document

Choose a citation style from the tabs below

Save time finding and organizing research with Mendeley

Sign up for free