Multiplex Genome Engineering Using CRISPR/Cas Systems

  • Cong Le, Ran F. Ann, Cox David, Lin Shuailiang, Barretto Robert, Habib Naomi, Hsu Patrick D., Wu Xuebing, Jiang Wenyan, Marraffini Luciano A. Z
N/ACitations
Citations of this article
10Readers
Mendeley users who have this article in their library.

Abstract

Functional elucidation of causal genetic variants and elements requires precise genome editing technologies. The type II prokaryotic CRISPR (clustered regularly interspaced short palindromic repeats)/Cas adaptive immune system has been shown to facilitate RNA-guided site-specific DNA cleavage. We engineered two different type II CRISPR/Cas systems and demonstrate that Cas9 nucleases can be directed by short RNAs to induce precise cleavage at endogenous genomic loci in human and mouse cells. Cas9 can also be converted into a nicking enzyme to facilitate homology-directed repair with minimal mutagenic activity. Lastly, multiple guide sequences can be encoded into a single CRISPR array to enable simultaneous editing of several sites within the mammalian genome, demonstrating easy programmability and wide applicability of the RNA-guided nuclease technology.

Cite

CITATION STYLE

APA

Cong Le, Ran F. Ann, Cox David, Lin Shuailiang, Barretto Robert, Habib Naomi, Hsu Patrick D., Wu Xuebing, Jiang Wenyan, Marraffini Luciano A., Z. F. (2006). Multiplex Genome Engineering Using CRISPR/Cas Systems, 445(June), 1504–1508.

Register to see more suggestions

Mendeley helps you to discover research relevant for your work.

Already have an account?

Save time finding and organizing research with Mendeley

Sign up for free