Multiplex {PCR} assay for species identification of bovine mastitis pathogens

  • Shome B
  • Mitra D
  • Bhuvana M
 et al. 
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Abstract

Aim: To develop and evaluate a multiplex {PCR} {(mPCR)} assay for simultaneous detection of 10 bacterial species causing bovine mastitis namely, Staphylococcus aureus, Staphylococcus chromogenes, Staphylococcus epidermidis, Staphylococcus sciuri, Staphylococcus haemolyticus, Staphylococcus simulans, Streptococcus agalactiae, Streptococcus dysgalactiae, Streptococcus uberis and Escherichia coli in milk. Methods and Results: A two-tube {mPCR} assay was developed. The accuracy of the {mPCR} was evaluated using 56 standard reference strains and 705 strains comprising of E.~coli (n~=~99), staphylococci (n~=~522) and streptococci (n~=~84). The threshold of detection of the {mPCR} assay was 10~fg of genomic {DNA} and {{

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Authors

  • B R Shome

  • Das S Mitra

  • M Bhuvana

  • N Krithiga

  • D Velu

  • R Shome

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