Multiplexed Toxin Analysis Using Four Golors of Quantum Dot Fluororeagents

  • Goldman E
  • Clapp A
  • Anderson G
 et al. 
  • 43

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Abstract

Quantum dots (QDs) have the potential to simplify the performance of multiplexed analysis. In this work, we prepared bioinorganic conjugates made with highly luminescent semiconductor nanocrystals (CdSe-ZnS core-shell QDs) and antibodies to perform multiplexed fluoroimmunoassays. Sandwich immunoassays for the detection of cholera toxin, ricin, shiga-like toxin 1, and staphylococcal enterotoxin B were performed simultaneously in single wells of a microtiter plate. Initially the assay performance for the detection of each toxin was examined. We then demonstrated the simultaneous detection of the four toxins from a single sample probed with a mixture of all four QD-antibody reagents. Using a simple linear equation-based algorithm, it was possible to deconvolute the signal from mixed toxin samples, which allowed quantitation of all four toxins simultaneously.

Author-supplied keywords

  • Biological
  • Biological: analysis
  • Biological: chemistry
  • Cadmium
  • Cadmium: chemistry
  • Fluorescent Antibody Technique
  • Fluorometry
  • Fluorometry: methods
  • Immunoconjugates
  • Immunoconjugates: chemistry
  • Immunoenzyme Techniques
  • Luminescent Measurements
  • Models
  • Protein Engineering
  • Protein Engineering: methods
  • Quantum Dots
  • Selenium
  • Selenium: chemistry
  • Semiconductors
  • Sulfides
  • Sulfides: chemistry
  • Toxins
  • Zinc Compounds
  • Zinc Compounds: chemistry

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Authors

  • Ellen R Goldman

  • Aaron R Aarsn R Clapp

  • George P Anderson

  • H Tetsuo Uyeda

  • J Matthew Mauro

  • Igor L Medintz

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