Multivesicular Release at Single Functional Synaptic Sites in Cerebellar Stellate and Basket Cells

  • Auger C
  • Kondo S
  • Marty A
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The purpose of the present work was to test the hypothesis that no more than one vesicle of transmitter can be liberated by an action potential at a single release site. Spontaneous and evoked IPSCs were recorded from interneurons in the molecular layer of cerebellar slices. Evoked IPSCs were obtained using either extracellular stimulation or paired recordings of presynaptic and postsynaptic neurons. Connections were identified as single-site synapses when evoked current amplitudes could be grouped into one peak that was well separated from the background noise. Peak amplitudes ranged from 30 to 298 pA. Reducing the release probability by lowering the external Ca2+ concentration or adding Cd2+failed to reveal smaller quantal components. Some spontaneous IPSCs (1.4–2.4%) and IPSCs evoked at single-site synapses (2–6%) were followed within

Author-supplied keywords

  • Animals
  • Cell Communication
  • Cerebellum
  • Cerebellum: metabolism
  • Electric Conductivity
  • GABA
  • Interneurons
  • Interneurons: metabolism
  • Interneurons: physiology
  • Neurotransmitter Agents
  • Neurotransmitter Agents: metabolism
  • Presynaptic Terminals
  • Presynaptic Terminals: physiology
  • Rats
  • Reaction Time
  • Reaction Time: physiology
  • Synapses
  • Synapses: physiology
  • Synaptic Transmission
  • Synaptic Transmission: physiology
  • Synaptic Vesicles
  • Synaptic Vesicles: metabolism
  • basket cells
  • quantal analysis
  • stellate cells
  • vesicular release

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  • Céline Auger

  • Satoru Kondo

  • Alain Marty

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