Systemic mycobacteriosis caused by Mycobacterium genavense was diagnosed
in a 7-year-old captive lineolated parakeet (Bolborhynchus lineola).
About a year before death, proventricular dilatation syndrome (PDS) was
suggested, because of persistent regurgitations and intermittent
diarrhoea. Necropsy examination did not show any signs typical of PDS
and mycobacterioses. No caseous necrosis, but focal ulcerated overgrowth
in the proventriculus, hypertrophy of intestinal mucosa and
splenomegaly, was found. Primary neoplasia was suspected. The crucial
examination was histopathology, which revealed changes typical of
mycobacteriosis and the presence of numerous acid-fast bacilli. A real-
time SYBR (R) Green PCR test was used and Mycobacterium genavense
infection was diagnosed. The mycobacterium was also cultured on BD
BACTEC (TM) 460TB 12B Middlebrook 7H12 medium.
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