Nanoscale mechanical and dynamical properties of DNA single molecules

  • Anselmi C
  • DeSantis P
  • Scipioni A
  • 1

    Readers

    Mendeley users who have this article in their library.
  • N/A

    Citations

    Citations of this article.

Abstract

Experimental evidence suggests DNA mechanical properties, in particular intrinsic curvature and flexibility, have a role in many relevant biological processes. Systematic investigations about the origin of DNA curvature and flexibility have been carried out; however, most of the applied experimental techniques need simplifying models to interpret the data, which can affect the results. Progress in the direct visualization of macromolecules allows the analysis of morphological properties and structural changes of DNAs directly from the digitised micrographs of single molecules. In addition, the statistical analysis of a large number of molecules gives information both on the local intrinsic curvature and the flexibility of DNA tracts at nanometric scale in relatively long sequences. However, it is necessary to extend the classical worm-like chain model (WLC) for des cribing conformations of intrinsically straight homogeneous polymers to DNA. This review describes the various methodologies proposed by different authors. © 2004 Elsevier B.V. All rights reserved.

Author-supplied keywords

  • DNA curvature
  • DNA flexibility
  • DNA-mica interactions
  • Divalent cations effects
  • Persistence length
  • Worm-like chain

Get free article suggestions today

Mendeley saves you time finding and organizing research

Sign up here
Already have an account ?Sign in

Find this document

Authors

  • C. Anselmi

  • P. DeSantis

  • A. Scipioni

Cite this document

Choose a citation style from the tabs below

Save time finding and organizing research with Mendeley

Sign up for free