Nanoscale tuning of enzyme localization for enhanced reactor performance in a novel magnetic-responsive biocatalytic membrane reactor

  • Gebreyohannes A
  • Bilad M
  • Verbiest T
 et al. 
  • 29


    Mendeley users who have this article in their library.
  • 10


    Citations of this article.


The synergistic magnetic interaction between biofunctionalized magnetic nanoparticles and a hybrid membrane is exploited to develop a nano-inspired, magnetic-responsive enzyme membrane (micro) reactor. The novelty of the process lies in the use of superparamagnetic nanoparticles both as enzyme carrier to form bionanocomposites and as nanofiller to form organic-inorganic (O/I) hybrid membrane to render both reversibly magnetizable. This reversible magnetic force facilitates dispersion of the enzymatically active magnetic nanoparticles (bionanocomposites) over the membrane surface, allows retention of the enzyme by a large pore, i.e., high-flux membrane and renders enzyme recovery after use is very easy. The feasibility and versatility of the concept is demonstrated through 2 case studies, i.e., a pectin/polygalacturonase and an arabinoxylan/xylanase system, for membrane fouling prevention through in-situ enzymatic membrane cleaning. This robust multidisciplinary approach resulted in a 75% reduction in filtration resistance, thus realizing significant energy savings and high reactor productivity. The advantages of the novel approach include: (i) absence of need for neither functionalized nor retentive membrane surfaces, (ii) no leakage of nanosized, high surface area immobilized enzymes through microporous membranes, (iii) full recovery and re-usability of the enzymes, (iv) possibility to apply enzyme cocktails to achieve optimal conversions and (v) use of the membrane beyond the enzyme life cycle.

Author-supplied keywords

  • Enzyme immobilization
  • Fouling
  • In-situ cleaning
  • Magnetic nanoparticles
  • Responsive membranes

Get free article suggestions today

Mendeley saves you time finding and organizing research

Sign up here
Already have an account ?Sign in

Find this document

Get full text


  • Abaynesh Yihdego Gebreyohannes

  • Muhammad Roil Bilad

  • Thierry Verbiest

  • Christophe M. Courtin

  • Emmie Dornez

  • Lidietta Giorno

Cite this document

Choose a citation style from the tabs below

Save time finding and organizing research with Mendeley

Sign up for free