This paper presents an investigation into magnetoelastic (ME) biosentinels that capture and detect low-concentration pathogenic bacteria in stagnant liquids. The ME biosentinels are designed to mimic a variety of white blood cell types, known as the main defensive mechanism in the human body against different pathogenic invaders. The ME biosentinels are composed of a freestanding ME resonator coated with an engineered phage that specifically binds with the pathogens of interest. These biosentinels are ferromagnetic and thus can be moved through a liquid by externally applied magnetic fields. In addition, when a time-varying magnetic field is applied, the ME biosentinels can be placed into mechanical resonance by magnetostriction. As soon as the biosentinels bind with the target pathogen through the phage-based biomolecular recognition, a change in the biosentinel’s resonant frequency occurs, and thereby the presence of the target pathogen can be detected. Detection of Bacillus anthracis spores under stagnant flow conditions was demonstrated.
CITATION STYLE
Horikawa, S., Chai, Y., Wikle, H. C., Dai, J., Hu, J., Suh, S.-J., … Chin, B. A. (2015). Nature-inspired magnetoelastic biosentinels for the detection of pathogenic bacteria in stagnant liquids. In Sensing for Agriculture and Food Quality and Safety VII (Vol. 9488, p. 94880C). SPIE. https://doi.org/10.1117/12.2087766
Mendeley helps you to discover research relevant for your work.