Neural differentiation of mouse embryonic stem cells on conductive nanofiber scaffolds

  • Kabiri M
  • Soleimani M
  • Shabani I
 et al. 
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Abstract

Nerve tissue engineering requires suitable precursor cells as well as the necessary biochemical and physical cues to guide neurite extension and tissue development. An ideal scaffold for neural regeneration would be both fibrous and electrically conductive. We have contrasted the growth and neural differentiation of mouse embryonic stem cells on three different aligned nanofiber scaffolds composed of poly l-lactic acid supplemented with either single- or multi-walled carbon-nanotubes. The addition of the nanotubes conferred conductivity to the nanofibers and promoted mESC neural differentiation as evidenced by an increased mature neuronal markers expression. We propose that the conductive scaffold could be a useful tool for the generation of neural tissue mimics in vitro and potentially as a scaffold for the repair of neural defects in vivo. © 2012 Springer Science+Business Media B.V.

Author-supplied keywords

  • Animals
  • Carbon
  • Carbon-nanotubes
  • Cell Culture Techniques
  • Cell Differentiation
  • Conductive nanofibers
  • Conductive scaffolds
  • Electrically conductive
  • Embryonic Stem Cells
  • Embryonic stem cells
  • In-vitro
  • In-vivo
  • L-Lactic acid
  • Lactic acid
  • Mammals
  • Mice
  • Mouse embryonic stem cells
  • Multi-walled
  • Nanofiber scaffold
  • Nanofibers
  • Nanotubes
  • Nerve tissue engineering
  • Neural defects
  • Neural differentiation
  • Neural differentiations
  • Neural regeneration
  • Neural tissue
  • Neurite extension
  • Neuronal markers
  • Neurons
  • Precursor cells
  • Scaffolds (biology)
  • Tissue
  • Tissue Engineering
  • Tissue Scaffolds
  • Tissue development
  • animal
  • article
  • cell differentiation
  • culture technique
  • embryonic stem cell
  • methodology
  • mouse
  • nanofiber
  • nerve cell
  • physiology
  • tissue engineering
  • tissue scaffold

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Authors

  • M Kabiri

  • M Soleimani

  • I Shabani

  • K Futrega

  • N Ghaemi

  • H H Ahvaz

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