A new method for determination of apparent kinetics parameters in recirculating packed-bed immobilized enzyme reactors

  • Özdural A
  • Tanyolaç D
  • Demircan Z
 et al. 
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Abstract

In this study, a simple and effective technique for characterizing Michaelis-Menten apparent kinetic parameters in packed-bed immobilized enzyme reactors is presented. The apparent kinetic parameters of immobilized glucose oxidase on weak base ion exchanger resin (Duolite A 568) were determined for different substrate flow rates in a recirculation system and compared with those for soluble glucose oxidase. It was observed that, for the experimental conditions, the immobilized enzyme Klmvalues in a packed-bed reactor were less than the soluble enzyme K1mvalue and were flow dependent. The value of Klmdecreased with increasing flow rate. © 2001 Elsevier Science Ltd. All rights reserved.

Author-supplied keywords

  • Ezyme
  • Immobilized glucose oxidase
  • Kinetics
  • Modelling
  • Packed-bed
  • Recirculation reactor

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