A new way of producing isomalto-oligosaccharide syrup by using the transglycosylation reaction of neopullulanase

  • Kuriki T
  • Yanase M
  • Takata H
 et al. 
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A new way of producing isomalto-oligosaccharide syrup from starch was developed. Isomalto-oligosaccharides contain one or more alpha-(1-->6)-glucosidic linkages with or without alpha-(1-->4)-glucosidic linkages. The isomalto-oligosaccharide syrups are receiving increased attention as food additives because it is thought that they help prevent dental caries and improve human intestinal microflora, acting as a growth factor for bifidobacteria. The new system for production of isomalto-oligosaccharide syrup is based on the strong alpha-(1-->6)-transglycosylation reaction of neopullulanase. Bacillus subtilis saccharifying alpha-amylase was simultaneously used with neopullulanase to improve the yield of isomalto-oligosaccharides. The yield of isomalto-oligosaccharides was increased to more than 60%, compared with a yield of 45.0% obtained by the conventional system. To reduce the costs, the use of immobilized neopullulanase was investigated. Almost the same yield of isomalto-oligosaccharides was obtained by using immobilized neopullulanase.

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  • SGR: 0027450658
  • PUI: 23096627
  • SCOPUS: 2-s2.0-0027450658
  • ISBN: 0099-2240
  • ISSN: 00992240
  • PMID: 16348919


  • T. Kuriki

  • M. Yanase

  • H. Takata

  • Y. Takesada

  • T. Imanaka

  • S. Okada

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