All current next-generation sequencing (NGS) platforms and applications require the sequencing library to have specific characteristics: in particular, size, size distribution, and 5' and 3' flanking sequences. This unit presents a robust protocol for converting a wide variety of input DNA samples into appropriate NGS libraries and discusses important considerations in experimental design, failure modes, and typical results. Curr. Protoc. Mol. Biol. 107:7.17.1-7.17.16. © 2014 by John Wiley & Sons, Inc.
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