Next-generation sequencing fragment library construction

  • Podnar J
  • Deiderick H
  • Hunicke-Smith S
  • 20


    Mendeley users who have this article in their library.
  • 0


    Citations of this article.


All current next-generation sequencing (NGS) platforms and applications require the sequencing library to have specific characteristics: in particular, size, size distribution, and 5' and 3' flanking sequences. This unit presents a robust protocol for converting a wide variety of input DNA samples into appropriate NGS libraries and discusses important considerations in experimental design, failure modes, and typical results. Curr. Protoc. Mol. Biol. 107:7.17.1-7.17.16. © 2014 by John Wiley & Sons, Inc.

Author-supplied keywords

  • Amplicon
  • Dna
  • Fragment
  • Libraries
  • Library
  • Ngs
  • Sequencing
  • Shotgun

Get free article suggestions today

Mendeley saves you time finding and organizing research

Sign up here
Already have an account ?Sign in

Find this document


  • Jessica Podnar

  • Heather Deiderick

  • Scott Hunicke-Smith

Cite this document

Choose a citation style from the tabs below

Save time finding and organizing research with Mendeley

Sign up for free