All current next-generation sequencing (NGS) platforms and applications require the sequencing library to have specific characteristics: in particular, size, size distribution, and 5' and 3' flanking sequences. This unit presents a robust protocol for converting awide variety of input DNA samples into appropriate NGS libraries and discusses important considerations in experimental design, failure modes, and typical results.
CITATION STYLE
Podnar, J., Deiderick, H., & Hunicke-Smith, S. (2014). Next-generation sequencing fragment library construction. Current Protocols in Molecular Biology, 2014, 7.17.1-7.17.16. https://doi.org/10.1002/0471142727.mb0717s107
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