Nocodazole induces mitotic cell death with apoptotic-like features in Saccharomyces cerevisiae

  • Endo K
  • Mizuguchi M
  • Harata A
 et al. 
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Abstract

We investigated the fate of budding yeast treated with nocodazole, a microtubule-depolymerizing drug. Cells died after mitotic arrest while staying in mitosis, suggesting that mitotic cell death, but not mitotic slippage, mainly occurs in nocodazole-treated cells. Nocodazole-treated cells showed features of apoptotic-like cell death, but not those of cell lysis or autophagy. Consistently, mitochondria-dependent production of reactive oxygen species was involved in the cell death. Similar cell death was also seen in cells after mitotic arrest by perturbation of the anaphase-promoting complex/cyclosome. In addition, caspase activity was found in nocodazole-treated cells, which was independent of the metacaspase, Mca1. Our results suggest that budding yeast can be a model to study mitotic cell death in cancer treatment with antimitotic drugs. © 2010 Federation of European Biochemical Societies.

Author-supplied keywords

  • Apoptosis
  • Budding yeast
  • Mitotic arrest
  • Mitotic cell death
  • Nocodazole

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Authors

  • Kingo Endo

  • Mayumi Mizuguchi

  • Aoi Harata

  • Go Itoh

  • Kozo Tanaka

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