Noninvasive imaging of intracellular lipid metabolism in macrophages by Raman microscopy in combination with stable isotopic labeling

  • Matthäus C
  • Krafft C
  • Dietzek B
 et al. 
  • 1


    Mendeley users who have this article in their library.
  • N/A


    Citations of this article.


Monocyte-derived macrophages play a key role in atherogenesis because their transformation into foam cells is responsible for deposition of lipids in plaques within arterial walls. The appearance of cytosolic lipid droplets is a hallmark of macrophage foam cell formation, and the molecular basics involved in this process are not well understood. Of particular interest is the intracellular fate of different individual lipid species, such as fatty acids or cholesterol. Here, we utilize Raman microscopy to image the metabolism of such lipids and to trace their subsequent storage patterns. The combination of microscopic information with Raman spectroscopy provides a powerful molecular imaging method, which allows visualization at the diffraction limit of the employed laser light and biochemical characterization through associated spectral information. In order to distinguish the molecules of interest from other naturally occurring lipids spectroscopically, deuterium labels were introduced. Intracellular distribution and metabolic changes were observed for serum albumin-complexed palmitic and oleic acid and cholesterol and quantitatively evaluated by monitoring the increase in CD scattering intensities at 0.5, 1, 3, 6, 24, 30, and 36 h. This approach may also allow for investigating the cellular trafficking of other molecules, such as nutrients, metabolites, and drugs. © 2012 American Chemical Society.

Author-supplied keywords

  • Arterial wall
  • Atherogenesis
  • Biochemical characterization
  • Body fluids
  • Cell Line
  • Cellular manufacturing
  • Cholesterol
  • Cholesterol Esters
  • Cytosolic
  • Deuterium
  • Diffraction
  • Diffraction limits
  • Fatty Acids
  • Foam Cells
  • Foam cells
  • Humans
  • Intracellular distribution
  • Isotopic labeling
  • Laser lights
  • Lipid Metabolism
  • Lipid droplets
  • Lipid metabolisms
  • Lipid species
  • Lipids
  • Macrophages
  • Metabolic changes
  • Metabolism
  • Microscopy
  • Molecular Imaging
  • Molecular imaging
  • Molecules
  • Monocyte-derived macrophages
  • Naturally occurring
  • Non-invasive imaging
  • Oleic acid
  • Physiology
  • Raman microscopy
  • Raman spectrometry
  • Raman spectroscopy
  • Scattering intensity
  • Spectral information
  • Spectrum Analysis, Raman
  • Triglycerides
  • article
  • cell line
  • cholesterol
  • cholesterol ester
  • cytology
  • fatty acid
  • foam cell
  • human
  • lipid
  • lipid metabolism
  • macrophage
  • metabolism
  • methodology
  • microscopy
  • molecular imaging
  • triacylglycerol

Get free article suggestions today

Mendeley saves you time finding and organizing research

Sign up here
Already have an account ?Sign in


  • C Matthäus

  • C Krafft

  • B Dietzek

  • B R Brehm

  • S Lorkowski

  • J Popp

Cite this document

Choose a citation style from the tabs below

Save time finding and organizing research with Mendeley

Sign up for free