Novel methods for biocatalyst screening

  • Wahler D
  • Reymond J
  • 86


    Mendeley users who have this article in their library.
  • 137


    Citations of this article.


There have been a number of recent advances in catalysis assays applicable for screening biocatalyst libraries in high-throughput format. These include instrumental assays such as high-performance liquid chromatography, mass spectrometry, capillary electrophoresis and IR-thermography, reagent-based assays producing spectroscopic signals (UV/VIS or fluorescence) in response to reaction progress, and assays based on fluorogenic or chromogenic substrates. These fluorogenic substrates enable the assaying of a variety of enzymes in enantioselective and stereoselective manner, including alcohol dehydrogenases, aldolases, lipases, amidases, epoxide hydrolases and phosphatases.

Get free article suggestions today

Mendeley saves you time finding and organizing research

Sign up here
Already have an account ?Sign in

Find this document


  • Denis Wahler

  • Jean Louis Reymond

Cite this document

Choose a citation style from the tabs below

Save time finding and organizing research with Mendeley

Sign up for free