There have been a number of recent advances in catalysis assays applicable for screening biocatalyst libraries in high-throughput format. These include instrumental assays such as high-performance liquid chromatography, mass spectrometry, capillary electrophoresis and IR-thermography, reagent-based assays producing spectroscopic signals (UV/VIS or fluorescence) in response to reaction progress, and assays based on fluorogenic or chromogenic substrates. These fluorogenic substrates enable the assaying of a variety of enzymes in enantioselective and stereoselective manner, including alcohol dehydrogenases, aldolases, lipases, amidases, epoxide hydrolases and phosphatases.
CITATION STYLE
Wahler, D., & Reymond, J. L. (2001, April 1). Novel methods for biocatalyst screening. Current Opinion in Chemical Biology. Elsevier Ltd. https://doi.org/10.1016/S1367-5931(00)00184-8
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