Novel peptides selected to bind vascular endothelial growth factor target the receptor-binding site

  • Fairbrother W
  • Christinger H
  • Cochran A
 et al. 
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Peptides that inhibit binding of vascular endothelial growth factor (VEGF) to its receptors, KDR and Flt-1, have been produced using phage display. Libraries of short disulfide-constrained peptides yielded three distinct classes of peptides that bind to the receptor-binding domain of VEGF with micromolar affinities. The highest affinity peptide was also shown to antagonize VEGF-induced proliferation of primary human umbilical vascular endothelial cells. The peptides bind to a region of VEGF known to contain the contact surface for Flt-1 and the functional determinants for KDR binding. This suggests that the receptor-binding region of VEGF is a binding "hot spot" that is readily targeted by selected peptides and supports earlier assertions that phage-derived peptides frequently target protein-protein interaction sites. Such peptides may lead to the development of pharmacologically useful VEGF antagonists.

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  • Wayne J. Fairbrother

  • Hans W. Christinger

  • Andrea G. Cochran

  • Germaine Fuh

  • Christopher J. Keenan

  • Clifford Quan

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