Novel use of poly(3,4-ethylenedioxythiophene) nanoparticles for fluorescent nucleic acid detection

  • Zhang Y
  • Liu S
  • Wang L
 et al. 
  • 20

    Readers

    Mendeley users who have this article in their library.
  • 8

    Citations

    Citations of this article.

Abstract

In this paper, we demonstrate the novel use of poly(3,4-ethylene dioxythiophene) (PEDOT) nanoparticle as a very effective fluorescent sensing platform for the detection of nucleic acid sequences. The principle of the assay lies in the fact that the adsorption of the fluorescently labeled single-stranded DNA (ssDNA) probe by PEDOT nanoparticle leads to substantial fluorescence quenching, followed by specific hybridization with the complementary region of the target DNA sequence. This results in desorption of the hybridized complex from PEDOT nanoparticle surface and subsequent recovery of fluorescence. A detection limit as low as 30 pM could be achieved in this sensing system. We also demonstrate its application for multiplexed detection of nucleic acid sequences. Furthermore, this sensing system can realize the detection of single-base mismatch even in multiplexed format. It is of importance to note that the successful use of this sensing platform in human blood serum system is also demonstrated.

Author-supplied keywords

  • blood serum
  • fluorescent
  • nanoparticles
  • nucleic acid detection
  • poly(3,4-ethylenedioxythiophene)

Get free article suggestions today

Mendeley saves you time finding and organizing research

Sign up here
Already have an account ?Sign in

Find this document

Authors

  • Abdullah M. AsiriCenter of Excellence for Advanced Materials Research - King Abdulaziz University

    Follow
  • Yingwei Zhang

  • Sen Liu

  • Lei Wang

  • Yonglan Luo

  • Jingqi Tian

Cite this document

Choose a citation style from the tabs below

Save time finding and organizing research with Mendeley

Sign up for free