Nucleotide sequence of the birA gene encoding the biotin operon represser and biotin holoenzyme synthetase functions of Escherichia coli

  • Howard P
  • Shaw J
  • Otsuka A
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Abstract

A 2.2-kb region of DNA containing the birA gene of Escherichia coli has been sequenced. The birA gene sequence predicts a 35.3-kDal [321 amino acids (aa)] bifunctional protein containing biotin-operon-repressor and biotin-holoenzyme-synthetase activities. Mutations, generated by random insertion of XhoI linkers, defined the extent of the gene. Mutations affecting one or more of five discernable properties of birA [Barker D. and Campbell A., J. Bacteriol., 143 (1980) 789-800] were mapped. Three mutations that result in temperature-sensitive (ts) growth, birA 85, birA215 and birA 879 mapped in the N-terminal two-thirds of the protein. The birA 352 mutation, which partially complements birA 215 and birA 879, maps in the N-terminal third of the protein. Finally, birA 3 61 maps closest to the amino terminus. ?? 1985.

Author-supplied keywords

  • Bacteriophage M13
  • deletion mapping
  • fatty acid synthesis
  • multifunctional protein
  • mutagenicity
  • oligonucleotide linker insertions
  • recombinant DNA

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Authors

  • Peter K. Howard

  • Janet Shaw

  • Anthony J. Otsuka

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