Nucleotide-mediated size fractionation of gold nanoparticles in aqueous solutions

  • Zhao W
  • Lin L
  • Hsing I
  • 3


    Mendeley users who have this article in their library.
  • 18


    Citations of this article.


It is well-known that the applications of nanoparticles are highly dependent on their size-related physical and chemical properties. Size fractionation, therefore, is important for the successful application of nanoparticles. In this study, we present a method for the size fractionation of gold nanoparticles (Au-nps) in aqueous solution, which combines the nucleotide-mediated stabilization and the size-dependent salt-induced aggregation of nanoparticles. With a coating layer of nucleotide, Au-nps undergo reversible salt-induced aggregation in aqueous solutions where the critical salt concentration (CSC) for the transition of monodispersed Au-nps to aggregated form is dependent on the size of nanoparticles; i.e., the smaller the particle, the higher is the CSC. Successful fractionations of a solution containing Au-nps of different sizes (10, 20, and 40 nm) were demonstrated with final purity of each fraction higher than 90%. Taking advantages of the rapidness of the nucleotide-mediated stabilization of Au-nps, the whole fractionation process can be completed within 1 h.

Get free article suggestions today

Mendeley saves you time finding and organizing research

Sign up here
Already have an account ?Sign in

Find this document


  • Wenting Zhao

  • Li Lin

  • I. Ming Hsing

Cite this document

Choose a citation style from the tabs below

Save time finding and organizing research with Mendeley

Sign up for free