Observation of microorganism colonies using a scanning-laser-beam pH-sensing microscope

  • Nakao M
  • Inoue S
  • Oishi R
 et al. 
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Abstract

The extracellular pH-distribution of colonies of Saccharomyces cerevisiae (yeast) and Escherichia coli (E. coli) were observed using a newly-developed scanning-laser-beam pH-sensing microscope. Colonies were incubated either on top of agarose plates or between the pH-sensing surface and the agar. In the latter case, colony growth was observed in-situ. The colonies could be observed within a period as short as 8 h for E. coli. The pH-distribution profiles by the colonies were found to be very sharp, in agreement with simulation results. © 1995.

Author-supplied keywords

  • colony-counting
  • metabolism
  • microorganism
  • pH sensor
  • pH-imaging sensor

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