Optimization of carotenoids extraction from Rhodobacter sphaeroides

  • Gu Z
  • Deming C
  • Yongbin H
 et al. 
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The comparison of the three methods ultrasonic assisting, grinding and HCl assisting on carotenoids extraction yield from Rhodobacter sphaeroides (R. sphaeroides) was carried out. The HCl-assisted extraction was found to be the most effective. And then, based on HCl-assisted extraction method, the effect of temperature, solvent-solid ratio and duration on the yield of carotenoids extracted from R. sphaeroides was investigated using single factor and Box-Behnken experimental design. The optimal conditions for HCl-assisted extraction of carotenoids from R. sphaeroides were found to be: temperature 30 °C, solvent-solid ratio 40 ml/g, duration 40 min. The first two extractions were enough to approximately exhaust the entire content of carotenoids in R. sphaeroides. © 2007 Swiss Society of Food Science and Technology.

Author-supplied keywords

  • Box-Behnken design
  • Carotenoids
  • Extraction
  • Optimization
  • Rhodobacter sphaeroides

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  • Zhenxin Gu

  • Chen Deming

  • Han Yongbin

  • Chen Zhigang

  • Gu Feirong

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