Optimization of carotenoids extraction from Rhodobacter sphaeroides

  • Glaeser J
  • Klug G
  • Allen J
 et al. 
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The comparison of the three methods ultrasonic assisting, grinding and HCl assisting on carotenoids extraction yield from Rhodobacter sphaeroides (R. sphaeroides) was carried out. The HCl-assisted extraction was found to be the most effective. And then, based on HCl-assisted extraction method, the effect of temperature, solvent-solid ratio and duration on the yield of carotenoids extracted from R. sphaeroides was investigated using single factor and Box-Behnken experimental design. The optimal conditions for HCl-assisted extraction of carotenoids from R. sphaeroides were found to be: temperature 30 °C, solvent-solid ratio 40 ml/g, duration 40 min. The first two extractions were enough to approximately exhaust the entire content of carotenoids in R. sphaeroides. © 2007 Swiss Society of Food Science and Technology.

Author-supplied keywords

  • (Rb. sphaeroides)
  • Alkyl and Aryl Transferases
  • Antenna
  • Bacterial photosynthesis
  • Blotting
  • Box-Behnken design
  • Carotenoid
  • Carotenoid bandshift
  • Carotenoids
  • Carotenoids: biosynthesis
  • Chromatophore
  • Electrophoresis
  • Extraction
  • Gel
  • Genetic Complementation Test
  • Geranylgeranyl-Diphosphate Geranylgeranyltransfera
  • Insertional
  • Light harvesting
  • Light harvesting complex
  • Membrane protein
  • Mutagenesis
  • Mutation
  • Operon
  • Optimization
  • Oxidoreductases
  • Oxidoreductases: genetics
  • Peptides
  • Peptides: analysis
  • Peptides: genetics
  • Photosynthetic Reaction Center Complex Proteins
  • Photosynthetic Reaction Center Complex Proteins: c
  • Photosynthetic Reaction Center Complex Proteins: m
  • Pigment-protein complex
  • Polyacrylamide Gel
  • Pulsed-Field
  • Reconstitution
  • Rhodobacter sphaeroides
  • Rhodobacter sphaeroides: genetics
  • Rhodobacter sphaeroides: metabolism
  • Rhodobacter sphaeroides: ultrastructure
  • Spectrophotometry
  • Synthetic biology
  • Transferases
  • Transferases: genetics
  • Ultraviolet
  • Western

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  • Jens Glaeser

  • Gabriele Klug

  • J P Allen

  • G Feher

  • T O Yeates

  • H Komiya

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