Optimization of carotenoids extraction from Rhodobacter sphaeroides

  • Glaeser J
  • Klug G
  • Allen J
 et al. 
  • 1

    Readers

    Mendeley users who have this article in their library.
  • N/A

    Citations

    Citations of this article.

Abstract

The comparison of the three methods ultrasonic assisting, grinding and HCl assisting on carotenoids extraction yield from Rhodobacter sphaeroides (R. sphaeroides) was carried out. The HCl-assisted extraction was found to be the most effective. And then, based on HCl-assisted extraction method, the effect of temperature, solvent-solid ratio and duration on the yield of carotenoids extracted from R. sphaeroides was investigated using single factor and Box-Behnken experimental design. The optimal conditions for HCl-assisted extraction of carotenoids from R. sphaeroides were found to be: temperature 30 °C, solvent-solid ratio 40 ml/g, duration 40 min. The first two extractions were enough to approximately exhaust the entire content of carotenoids in R. sphaeroides. © 2007 Swiss Society of Food Science and Technology.

Author-supplied keywords

  • (Rb. sphaeroides)
  • Alkyl and Aryl Transferases
  • Antenna
  • Bacterial photosynthesis
  • Blotting
  • Box-Behnken design
  • Carotenoid
  • Carotenoid bandshift
  • Carotenoids
  • Carotenoids: biosynthesis
  • Chromatophore
  • Electrophoresis
  • Extraction
  • Gel
  • Genetic Complementation Test
  • Geranylgeranyl-Diphosphate Geranylgeranyltransfera
  • Insertional
  • Light harvesting
  • Light harvesting complex
  • Membrane protein
  • Mutagenesis
  • Mutation
  • Operon
  • Optimization
  • Oxidoreductases
  • Oxidoreductases: genetics
  • Peptides
  • Peptides: analysis
  • Peptides: genetics
  • Photosynthetic Reaction Center Complex Proteins
  • Photosynthetic Reaction Center Complex Proteins: c
  • Photosynthetic Reaction Center Complex Proteins: m
  • Pigment-protein complex
  • Polyacrylamide Gel
  • Pulsed-Field
  • Reconstitution
  • Rhodobacter sphaeroides
  • Rhodobacter sphaeroides: genetics
  • Rhodobacter sphaeroides: metabolism
  • Rhodobacter sphaeroides: ultrastructure
  • Spectrophotometry
  • Synthetic biology
  • Transferases
  • Transferases: genetics
  • Ultraviolet
  • Western

Get free article suggestions today

Mendeley saves you time finding and organizing research

Sign up here
Already have an account ?Sign in

Find this document

Authors

  • Jens Glaeser

  • Gabriele Klug

  • J P Allen

  • G Feher

  • T O Yeates

  • H Komiya

Cite this document

Choose a citation style from the tabs below

Save time finding and organizing research with Mendeley

Sign up for free