p54nrb associates with the 5??? splice site within large transcription/splicing complexes

  • Kameoka S
  • Duque P
  • Konarska M
  • 58

    Readers

    Mendeley users who have this article in their library.
  • 122

    Citations

    Citations of this article.

Abstract

The functional coupling of transcription and splicing has been reported both in vivo and in vitro, but the molecular mechanisms governing these interactions remain largely unknown. Here we show that p54(nrb), a transcription/splicing factor, associates with the 5' splice site (SS) within large complexes present in HeLa cell nuclear extracts, in which the hyperphosphorylated form of RNA polymerase II (RNAPIIO) is associated with U1 or U1 and U2 snRNPs. These RNAPIIO-snRNP complexes also contain other transcription/splicing factors, such as PSF and TLS, as well as transcription factors that interact with RNAPIIO during elongation, including P-TEFb, TAT-SF1 and TFIIF. The presence of these factors in functional elongation complexes, demonstrated using an immobilized DNA template assay, strongly suggests that the RNAPIIO-snRNP complexes reflect physiologically relevant interactions between the transcription and splicing machineries. Our finding that both p54(nrb) and PSF, which bind the C-terminal domain of the largest subunit of RNAPII, can interact directly with the 5' SS indicates that these factors may mediate contacts between RNAPII and snRNPs during the coupled transcription/splicing process.

Author-supplied keywords

  • PSF
  • RNA polymerase II
  • Splicing complexes
  • Transcription
  • p54nrb

Get free article suggestions today

Mendeley saves you time finding and organizing research

Sign up here
Already have an account ?Sign in

Find this document

Authors

  • Sei Kameoka

  • Paula Duque

  • Maria M. Konarska

Cite this document

Choose a citation style from the tabs below

Save time finding and organizing research with Mendeley

Sign up for free