The use of palmitate-conjugated protein A for coating cells with artificial receptors which facilitate intercellular interactions

  • Kim S
  • Peacock J
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A method is described in which palmitate-conjugated protein A (pal-protein A) incorporated onto cell membranes is used to anchor antibodies on the surface of cells. Pal-protein A was stabilized on the cell surfaces via insertion of the hydrophobic palmitate moieties into the phospholipid bilayer of the plasma membrane. This membrane-associated pal-protein A retained its binding affinity for the Fc region of rabbit immunoglobulin G (IgG) molecules. Using this system, cells can be coated with antibody (Ab) molecules of a desired specificity that can serve as artificial receptors for soluble or cell surface antigens (Ags). In this report, we show that A22.E10 T hybridoma cells coated with rabbit anti-mouse IgG (RaMIgG) exhibited increased adhesiveness to surface IgG positive mouse B cells. A high level of heterotypic intercellular conjugation was observed in cells coated with specific artificial receptors as compared to cells coated with nonspecific artificial receptors. © 1993.

Author-supplied keywords

  • Artificial receptor
  • Cell-cell interaction
  • Palmitate-conjugated protein
  • Protein A

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  • Stanley A. Kim

  • James S. Peacock

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