Partial purification and characterization of a polymannuronic acid depolymerase produced by a mucoid strain of Pseudomonas aeruginosa isolated from a patient with cystic fibrosis

  • Dunne Jr. W
  • Buckmire F
  • 1

    Readers

    Mendeley users who have this article in their library.
  • N/A

    Citations

    Citations of this article.

Abstract

An exopolysaccharide depolymerase was isolated from a mucoid strain of Pseudomonas aeruginosa of cystic fibrosis origin. Purified preparations of the depolymerase showed maximum activity against the unacetylated polymannuronic acid exopolysaccharide from the same strain and little activity against commercially prepared alginic acid. The evidence suggests that the enzyme is either periplasmic in location or associated with the outer cell membrane and is released extracellularly, in the absence of cell lysis, after a reduction of the culture magnesium (Mg2+) concentration below 3.0 mM. The depolymerase is also released after the addition of sublethal concentrations of EDTA to cultures containing 3.0 mM Mg2+. A survey of additional mucoid P. aeruginosa isolates recovered from patients with cystic fibrosis showed that nearly 60% demonstrated similar depolymerase activity while none of the nonmucoid revertants of the parent strains produced detectable depolymerase activity.

Author-supplied keywords

  • Alginates/metabolism
  • Cell Membrane/enzymology
  • Cystic Fibrosis/*microbiology
  • Edetic Acid/pharmacology
  • Glucosephosphate Dehydrogenase/metabolism
  • Glucuronic Acid
  • Glycoside Hydrolases/*isolation & purification/met
  • Hexuronic Acids
  • Humans
  • Kinetics
  • Magnesium/pharmacology
  • Molecular Weight
  • Potassium Chloride/pharmacology
  • Pseudomonas Infections/microbiology
  • Pseudomonas aeruginosa/*enzymology/isolation & pur
  • Sodium Chloride/pharmacology
  • Substrate Specificity

Get free article suggestions today

Mendeley saves you time finding and organizing research

Sign up here
Already have an account ?Sign in

Find this document

Authors

  • W M Dunne Jr.

  • F L Buckmire

Cite this document

Choose a citation style from the tabs below

Save time finding and organizing research with Mendeley

Sign up for free