Partial sequencing and expression of genes involved in glucose metabolism in adipose tissues and skeletal muscle of healthy cats

  • Zini E
  • Linscheid P
  • Franchini M
 et al. 
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Abstract

Impaired insulin sensitivity is increasingly recognised in cats, but sequences of genes involved in insulin-signalling are largely undetermined in this species. In this study, extended feline mRNA sequences were determined for the adiponectin, glucose transporter-1 (GLUT1), GLUT4, peroxisome proliferative activated receptor-gamma1 (PPARgamma1), PPARgamma2, plasminogen activator inhibitor-1 (PAI-1), monocyte chemoattractant protein-1 (MCP-1) and insulin receptor genes. Conserved dog-specific primers identified from human-dog mRNA alignments were used to amplify feline cDNA in the polymerase chain reaction (PCR). The feline sequences determined by this method were used to design feline-specific primers suitable for real-time PCR for quantification of gene expression in insulin sensitive tissues of healthy cats. Partial sequences of feline mRNAs had 86-95% identity with dog and human genes. Expression of adiponectin, GLUT1, GLUT4, PPARgamma1, PPARgamma2, PAI-1 and insulin receptor mRNA was detected and quantified in subcutaneous and visceral fat and skeletal muscle, whereas MCP-1 mRNA was detected in adipose tissue but not in skeletal muscle. Further characterisation of genes related to glucose metabolism in cats will provide additional insights into insulin-signalling mechanisms in this species.

Author-supplied keywords

  • Adipose Tissue/*metabolism
  • Animals
  • Blood Glucose/*metabolism
  • Cats/genetics/*metabolism
  • Energy Metabolism/*genetics
  • Female
  • Gene Expression/genetics
  • Glucose Transport Proteins, Facilitative/genetics
  • Insulin Resistance/*genetics
  • Insulin/blood
  • Male
  • Muscle, Skeletal/*metabolism
  • PPAR gamma/genetics
  • Receptor, Insulin/genetics
  • Species Specificity

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Authors

  • E Zini

  • P Linscheid

  • M Franchini

  • K Kaufmann

  • E Monnais

  • A P Kutter

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