Partitioning, diffusivity and clearance of skin permeants in mammalian dermis

  • Kretsos K
  • Miller M
  • Zamora-Estrada G
 et al. 
  • 35


    Mendeley users who have this article in their library.
  • 50


    Citations of this article.


The partition coefficients (Kde) and diffusivities (Dde) of compounds in mammalian dermis were examined through an analysis of in vitro permeation data obtained from the literature combined with experimental results with the test permeant,3H-testosterone. The literature data involved 26 compounds ranging in molecular weight from 18 to 476 Da and four species-human, guinea pig, rat and mouse. Testosterone was studied by permeation and desorption measurements employing excised human dermis in the presence and absence of external serum albumin. Mathematical models for both Kdeand Ddewere developed. The Kdemodel involved ionization, binding to extravascular serum proteins and partitioning into a small lipid compartment. The Ddemodel employed a free diffusivity with a liquid-like size dependence multiplied by a binding factor derived from Kde. An additional analysis considered in vivo dermal concentration profiles of topically applied permeants. Literature data for 5 of 6 permeants were shown to be well described by a previously published model for capillary clearance in the dermis, which leads to an exponential decay of concentration with depth. Computed decay lengths (1/e values) ranged from 210 to 920 μm, and the corresponding clearance rate constants kderanged from 0.9 × 10-4to 14 × 10-4s-1(n = 8). Departures from the exponential decay profile are discussed in terms of non-uniform capillary clearance and incomplete attainment of a steady-state. © 2007 Elsevier B.V. All rights reserved.

Author-supplied keywords

  • In silico prediction
  • Mathematical model
  • Percutaneous absorption
  • Skin
  • Topical delivery
  • Transdermal

Get free article suggestions today

Mendeley saves you time finding and organizing research

Sign up here
Already have an account ?Sign in

Find this document


Cite this document

Choose a citation style from the tabs below

Save time finding and organizing research with Mendeley

Sign up for free