Pathogenicity of Vibrio alginolyticus for cultured gilt-head sea bream (Sparus aurata L.)

  • Balebona M
  • Andreu M
  • Bordas M
 et al. 
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Abstract

The in vivo and in vitro pathogenic activities of whole cells and extracellular products of Vibrio alginolyticus for cultured gilt-head sea bream were evaluated. The 50% lethal doses ranged from 5.4 x 104 to 1.0 x 106 CFU/g of body weight. The strains examined had the ability to adhere to skin, gill, and intestinal mucus of sea bream and to cultured cells of a chinook salmon embryo cell line. In addition, the in vitro ability of V. alginolyticus to adhere to mucus and skin cells of sea bream was demonstrated by scanning electron microscopy. The biological activities of extracellular products of V. alginolyticus were hydrolytic activities; the products were able to degrade sea bream mucus. V. alginolyticus was cytotoxic for fish cell lines and lethal for sea bream. Moreover, the extracellular products could degrade sea bream tissues. However, experiments performed with the bath immersion inoculation technique demonstrated that V. alginolyticus should be considered a pathogen for sea bream only when the mucus layer is removed and the skin is damaged.

Author-supplied keywords

  • Animals
  • Archosargus rhomboidalis
  • Bacteria (microorganisms)
  • Bacterial Adhesion
  • Bacterial Toxins
  • Cell-Free System
  • Erythrocytes
  • Fish Diseases
  • Gills
  • Hemagglutination Tests
  • Horses
  • Hyperoglyphe porosa
  • Intestinal Mucosa
  • Muscle, Skeletal
  • Oncorhynchus tshawytscha
  • Perciformes
  • Posibacteria
  • Skin
  • Sparus aurata
  • Vibrio
  • Vibrio Infections
  • Virulence
  • article
  • bacterial virulence
  • bacterium adherence
  • cytotoxicity
  • fish
  • in vitro study
  • in vivo study
  • inoculation
  • mariculture
  • nonhuman
  • pathogenicity
  • skin cell
  • tissue necrosis
  • vibrio alginolyticus
  • viral disease

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Authors

  • M C Balebona

  • M J Andreu

  • M A Bordas

  • I Zorilla

  • M A Moriñgo

  • J J Borrego

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