Performance of an immobilized fuculose-1-phosphate aldolase for stereoselective synthesis

  • Suau T
  • Álvaro G
  • Benaiges M
 et al. 
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Abstract

A derivative of fuculose-1-phosphate aldolase, immobilized with high loading on glyoxal-agarose gels, has been characterized and evaluated as a biocatalyst for an aldol addition reaction. The reaction of the solid biocatalyst was diffusion-controlled for conversion of its natural substrate. Nevertheless, when catalyzing the synthesis of a biologically active aminopolyol, the lower reaction rate with non-natural substrates led to a process controlled by the intrinsic enzyme kinetics. The resulting biocatalyst has high synthetic specific activity and has been successfully used in batch synthesis reactions with high conversion. In addition, the immobilized aldolase has been employed in fed-batch synthesis, increasing the selectivity of the reaction and obtaining high conversion (88%).

Author-supplied keywords

  • Aldol addition
  • Diffusional limitations
  • Fuculose-1-phosphate aldolase
  • Immobilized enzymes
  • Specific activity

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Authors

  • Gregorio AlvaroUniversitat Autonoma de Barcelona Escola d'Enginyeria

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  • Trinitat Suau

  • M. Dolors Benaiges

  • Josep López-Santín

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