Persistence of asthma requires multiple feedback circuits involving type 2 innate lymphoid cells and IL-33

  • Christianson C
  • Goplen N
  • Zafar I
 et al. 
  • 88


    Mendeley users who have this article in their library.
  • 95


    Citations of this article.


Background: Asthma in a mouse model spontaneously resolves after cessation of allergen exposure. We developed a mouse model in which asthma features persisted for 6 months after cessation of allergen exposure. Objective: We sought to elucidate factors contributing to the persistence of asthma. Methods: We used a combination of immunologic, genetic, microarray, and pharmacologic approaches to dissect the mechanism of asthma persistence. Results: Elimination of T cells though antibody-mediated depletion or lethal irradiation and transplantation of recombination-activating gene (Rag1)-/-bone marrow in mice with chronic asthma resulted in resolution of airway inflammation but not airway hyperreactivity or remodeling. Elimination of T cells and type 2 innate lymphoid cells (ILC2s) through lethal irradiation and transplantation of Rag2-/-γc-/-bone marrow or blockade of IL-33 resulted in resolution of airway inflammation and hyperreactivity. Persistence of asthma required multiple interconnected feedback and feed-forward circuits between ILC2s and epithelial cells. Epithelial IL-33 induced ILC2s, a rich source of IL-13. The latter directly induced epithelial IL-33, establishing a positive feedback circuit. IL-33 autoinduced, generating another feedback circuit. IL-13 upregulated IL-33 receptors and facilitated IL-33 autoinduction, thus establishing a feed-forward circuit. Elimination of any component of these circuits resulted in resolution of chronic asthma. In agreement with the foregoing, IL-33 and ILC2 levels were increased in the airways of asthmatic patients. IL-33 levels correlated with disease severity. Conclusions: We present a critical network of feedback and feed-forward interactions between epithelial cells and ILC2s involved in maintaining chronic asthma. Although T cells contributed to the severity of chronic asthma, they were redundant in maintaining airway hyperreactivity and remodeling.

Author-supplied keywords

  • Chronic asthma
  • Feedback circuit
  • IL-33
  • T cells
  • Type 2 innate lymphoid cells

Get free article suggestions today

Mendeley saves you time finding and organizing research

Sign up here
Already have an account ?Sign in

Find this document


  • Christina A. Christianson

  • Nicholas P. Goplen

  • Iram Zafar

  • Chaoyu Irvin

  • James T. Good

  • Donald R. Rollins

Cite this document

Choose a citation style from the tabs below

Save time finding and organizing research with Mendeley

Sign up for free