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Phosphate solubilizing bacteria and their role in plant growth promotion

Rodríguez H, Fraga R, Restrepo Franco G, Marulanda Moreno S, De la Fe Pérez Y, Díaz de la Osa A, Lucia Baldani V, Hernández Rodríguez A, Estrada G, Baldani V, de Oliveira D, Urquiaga S, Baldani J, Marschner P, Crowley D, Rengel Z, Alikhani H, Saleh-Rastin N, Antoun H, Zaimes G, Schultz R, La P, Vegetal V, Xiao C, Chi R, Hu L, Chuang C, Kuo Y, Chao C, Chao W, Liu J, Yang J, Liang X, Zhao Y, Cade-Menun B, Hu Y, Bashan Y, Kamnev A, de-Bashan L, Bishnoi U, Kumar V, Narula N, Henri F, Laurette N, Annette D, John Q, Merbach W, Fankem H, Deubel A, Singh A, Parmar N, Kuhad R, Ward O, Kaur G, Reddy M, Liu Z, Li Y, Zhang S, Fu Y, Fan X, Patel J, Zhang M, Ghosh R, Barman S, Mukherjee R, Mandal N, Madhaiyan M, Saravanan V, Jovi D, Lee H, Thenmozhi R, Hari K, Sa T, Behera B, Singdevsachan S, Mishra R, Dutta S, Thatoi H, Azziz G, Bajsa N, Haghjou T, Taul?? C, Valverde ?, Igual J, Arias A, Taktek S, Tr??panier M, Servin P, St-Arnaud M, Pich?? Y, Fortin J, Antoun H, Nautiyal C ...see all

Biology and Fertility of Soils, vol. 43, issue 5 (1999) p. 319–339 Published by Elsevier B.V.

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Two of 187 fungal isolates (Aspergillus niger 1B and 6A) displaying superior phosphate (P) solubilization and hydrolytic enzyme secretion were studied using P forms of calcium (Ca-P), iron (Fe-P), and aluminum (Al-P). Phosphate solubilization in a sucrose-basal salt (SB) broth was increased and pH decreased by both isolates. In Ca-P medium, solubilization for 6A was approximately 322 mu g P mL(-1) and pH decreased by 4.2 units to 2.3 in 72 h. However, when pH value of the SB broth was lowered to 2.5 using HCl, 65.3 +/- 0.4 mu g mL(-1) of P was released from Ca-P, whereas trace amounts of P were released from Fe-P and Al-P. Both isolates displayed enhanced Al-P solubilization using NH4Cl rather than KNO3 as the N source; final pH values were not significantly different. With Ca-P, gluconic acid was predominantly produced by 1B and 6A, whereas oxalic acid predominated with Fe-P and Al-P. Addition of gluconic acid (final concentration of 8.5 mu mol mL(-1)) to Ca-P-supplemented SB lowered pH (2.9) and solubilized phosphate (146.0 +/- 1.0 mu g mL(-1)). Similarly, addition of oxalic acid (final concentration 6.6 mu mol mL(-1)) to Ca-P- and Fe-P-amended media solubilized P (60.2 +/- 0.9 and 21.6 +/- 2.1 mu g mL(-1), respectively), although these quantities were significantly lower than those detected in unamended SB. The presence of unidentified P solubilized compound(s) in the dialyzed (MW > 500) supernatant warrants further study. In pot experiments, significant increases in plant (Brassica chinensis Linn.) dry weight and N and P contents were observed with the addition of isolate 6A, when a small amount of organic fertilizer together with either rock phosphate (South African apatite) or Ca-P served as the main P sources.

Author-supplied keywords

  • AMF extraradical hyphae
  • Aspergillus niger
  • Azotobacter chroococcum
  • Bacillus
  • Bacterial inoculation
  • Bacterias promotoras de creciemiento vegetal
  • Biofertilizer
  • Burkholderia
  • Calcareous soils
  • Calcium phosphate
  • Crop-pasture rotations
  • Diammonium phosphate
  • G. diazotrophicus
  • Glomus irregulare
  • Growth emergence
  • Hyphosphere
  • Indole acetic acid
  • Indole production
  • Inorganic phosphate solubilization
  • Inositol hexaphosphate
  • Legumes
  • Mangrove
  • Microbiological growth medium
  • Microorganisms
  • Nitrogen use efficiency
  • Nitrogenase activity
  • No-tillage
  • Organic acids
  • Oryza sativa
  • PGPR
  • PSB
  • Pantoea cypripedii
  • Penicillium oxalicum
  • Penicillium spp.
  • Phosphatases
  • Phosphate rock solubilization
  • Phosphate solubilization
  • Phosphate solubilization ability
  • Phosphate solubilizer
  • Phosphate solubilizing bacteria
  • Phosphate solubilizing bacteria (PSB)
  • Phosphate solubilizing growth medium
  • Phosphate-solubilizing bacteria
  • Phosphorous and zinc solubilization
  • Phosphorus mineralization
  • Phytohormone production
  • Plant growth promoting bacteria
  • Pseudomonas
  • Pseudomonas plecoglossicida
  • Rhizobacteria
  • Rhizobium
  • Rhizosphere
  • Soil bacterial diversity
  • Soil fertility
  • Soil management
  • Solubilización de fosfato
  • Sustainable agriculture
  • Tricalcium phosphate
  • Wheat
  • Yield
  • a
  • acc deaminase
  • ahmad
  • aligarh muslim
  • aluminum phosphate
  • carboxylic acids
  • department of agricultural microbiology
  • e
  • faculty of agricultural sciences
  • growth regulators
  • khan
  • kinetics of solubilization
  • lycopodium cernuum l
  • m
  • mineralization
  • organic acids
  • pH
  • phosphate solubilization
  • phosphate solubilizing bacteria
  • plant
  • plant growth
  • pseudomonas fluorescens
  • s
  • solubilization
  • soluble phosphate
  • zaidi

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  • H Rodríguez

  • R Fraga

  • Gloria María Restrepo Franco

  • Sandra Marulanda Moreno

  • Yeised De la Fe Pérez

  • Acela Díaz de la Osa

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