Phosphoproteomics: Searching for a needle in a haystack

  • Tichy A
  • Salovska B
  • Rehulka P
 et al. 
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Most of the cellular processes are regulated by reversible phosphorylation of proteins, which in turn plays a critical role in the regulation of gene expression, cell division, signal transduction, metabolism, differentiation, and apoptosis. Mass spectrometry of phosphopeptides obtained from tryptic protein digests has become a powerful tool for characterization of phosphoproteins involved in these processes. However, there is a general need to significantly enrich the phosphopeptide content to compensate their low abundance, insufficient ionization, and suppression effects of non-phosphorylated peptides.This paper aims to give a comprehensive overview on the methods involved in recent phosphoproteomics. It presents a description of contemporary enrichment techniques with references to particular studies and compares different approaches to characterization of phosphoproteome by mass spectrometry. © 2011 Elsevier B.V.

Author-supplied keywords

  • MS/MS
  • Phosphopeptide enrichment
  • Quantitative phosphoproteomics

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  • Ales Tichy

  • Barbora Salovska

  • Pavel Rehulka

  • Jana Klimentova

  • Jirina Vavrova

  • Jiri Stulik

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