Photobleaching correction in fluorescence microscopy images

  • Vicente N
  • Diaz Zamboni J
  • Adur J
 et al. 
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Abstract

Fluorophores are used to detect molecular expression by highly specific antigen-antibody reactions\r in fluorescence microscopy techniques. A portion of the fluorophore emits fluorescence when\r irradiated with electromagnetic waves of particular wavelengths, enabling its detection.\r Photobleaching irreversibly destroys fluorophores stimulated by radiation within the excitation\r spectrum, thus eliminating potentially useful information. Since this process may not be completely\r prevented, techniques have been developed to slow it down or to correct resulting alterations\r (mainly, the decrease in fluorescent signal). In the present work, the correction by photobleaching\r curve was studied using E-cadherin (a cell-cell adhesion molecule) expression in Bufo arenarum\r embryos. Significant improvements were observed when applying this simple, inexpensive and fast\r technique.

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