Phylogenetic compositions of bacterioplankton from two California estuaries compared by denaturing gradient gel electrophoresis of 16S rDNA fragments

  • Murray A
  • Hollibaugh J
  • Orrego C
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Abstract

The phylogenetic compositions of bacterioplankton assemblages from San Francisco Bay and Tomales Bay, Calif., differed substantially when analyzed by PCR-denaturing gradient gel electrophoresis; these differences are consistent with the results of previous studies demonstrating differences in their metabolic capabilities. PCR-denaturing gradient gel electrophoresis analysis of complex microbial assemblages was sensitive and reliable, and the results were reproducible as shown by experiments with constructed and naturally occurring assemblages.

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  • PMID: 8779608
  • SGR: 0030036446
  • ISBN: 0099-2240
  • SCOPUS: 2-s2.0-0030036446
  • ISSN: 00992240
  • PUI: 26235282

Authors

  • Alison E. Murray

  • James T. Hollibaugh

  • Cristián Orrego

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