Plasma malondialdehyde as biomarker of lipid peroxidation: Effects of acute exercise

  • Spirlandeli A
  • Deminice R
  • Jordao A
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Abstract

The marker most frequently used to indicate the level of lipid peroxidation in the field of exercise and sports is malondialdehyde (MDA), which can be determined by many different techniques. However, there are few studies discussing differences and advantages of the methods for MDA assay in sports science field. The aim of the present study was to compare three techniques for quantification of MDA in plasma of humans subjected to acute exercise. MDA was determined by high performance liquid chromatography (MDA-HPLC), thiobarbituric acid reactive species (MDA-TBARS) and 1-methyl-2-phenylindole (MDA-MP) techniques in the plasma of 8 healthy male soccer athletes before and after acute exercise. Acute exercise significantly increased (P

Author-supplied keywords

  • HPLC
  • TBARS
  • acute exercise
  • lipid peroxidation
  • malondialdehyde

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