Plasmacytoid dendritic cells (PDC) are the major DC subset innately producing cytokines in human lymph nodes.

  • Cox K
  • North M
  • Burke M
 et al. 
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Abstract

Plasmacytoid dendritic cells (PDC) constitute a distinct subset of DC found in human peripheral lymph nodes (LN), but little is known about their function. Cell suspensions were prepared from tumor draining LN (n=20) and control LN (n=11) of women undergoing surgical resection for primary breast cancer and elective surgery for benign conditions, respectively. Using four-color flow cytometry, human leukocyte antigen-DR+ DC subsets were identified phenotypically. The proportions and numbers of cells innately producing interleukin (IL)-4, IL-10, IL-12, and interferon-gamma (IFN-gamma) were also measured from intracellular accumulation of cytokine after blocking with monensin. All flow cytometry data were collected without compensation and were compensated off-line using the Winlist algorithm (Verity software). This package also provided the subtraction program to calculate percentage positive cells and intensity of staining. PDC (CD11c-, CD123+) expressed more cytokines than did myeloid DC (CD11c+) or CD1a+ putative "migratory" DC (P

Author-supplied keywords

  • Adult
  • Aged
  • Breast Neoplasms
  • Breast Neoplasms: immunology
  • Breast Neoplasms: secondary
  • Cytokines
  • Cytokines: analysis
  • Cytokines: biosynthesis
  • Cytokines: immunology
  • Dendritic Cells
  • Dendritic Cells: cytology
  • Dendritic Cells: immunology
  • Female
  • Flow Cytometry
  • Humans
  • Immunity, Innate
  • Immunity, Innate: immunology
  • Interferon-gamma
  • Interferon-gamma: biosynthesis
  • Interferon-gamma: immunology
  • Interleukin-10
  • Interleukin-10: biosynthesis
  • Interleukin-10: immunology
  • Interleukin-12
  • Interleukin-12: biosynthesis
  • Interleukin-12: immunology
  • Interleukin-4
  • Interleukin-4: biosynthesis
  • Interleukin-4: immunology
  • Lymph Nodes
  • Lymph Nodes: cytology
  • Lymph Nodes: immunology
  • Lymph Nodes: pathology
  • Lymphatic Metastasis
  • Middle Aged
  • Tumor Cells, Cultured
  • Tumor Escape
  • Tumor Escape: immunology

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Authors

  • Karina Cox

  • Margaret North

  • Michael Burke

  • Hemant Singhal

  • Sophie Renton

  • Nayef Aqel

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